mastercycler nexus gradient (Eppendorf AG)

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Eppendorf AG mastercycler nexus gradient
Mastercycler Nexus Gradient, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200034 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eppendorf+mastercycler+gradient/Eppendorf/pmc13066795-103-56-62
Average 99 stars, based on 200034 article reviews

mastercycler nexus gradient - by Bioz Stars, 2026-09

99/100 stars

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Reverse Transcription:

Article Title: Organelle Sorting and Proteomic Analysis to Identify Proteins Involved in the Uptake and Intracellular Trafficking of Nanoparticles
Article Snippet: .. Next, the total mRNA was extracted from the cells recovered from four replicate wells using a Maxwell 16 simplyRNA Cells Kit (Promega), following the manufacturer's instructions. cDNA was prepared in an Eppendorf Mastercycler gradient by reverse transcription from 0.2 μg RNA using a M‐MLV reverse transcripase (Promega) following manufacturer's instructions. .. The cycle which was followed was: 20 °C for 10 min, 42 °C for 30 min, 20 °C for 12 min, 99 °C for 5 min, and 20 °C for 5 min. Quantitative RT‐PCR was then used to quantify the gene expression levels (10 nm cDNA per sample, 4 replicate wells per target) using a SensiMix SYBR kit (Bioline) in a ABI7900HT sequence detection system (Applied Biosystems).

Amplification:

Article Title: Soil Biodiversity of Eucalyptus saligna: Insights Into Bacterial and Nematode Communities.
Article Snippet: .. Amplification was conducted in an Eppendorf Mastercycler Gradient, with the cycling profile consisting of an initial denaturation at 94°C for 3 min; 37 cycles of denaturation at 94°C for 45 s, annealing at 50°C for 45 s and extension at 72°C for 45 s; followed by a final elongation step at 72°C for 6 min and a hold at 4°C. ..

Article Title: Soil Biodiversity of Eucalyptus saligna : Insights Into Bacterial and Nematode Communities
Article Snippet: .. Amplification was conducted in an Eppendorf Mastercycler Gradient, with the cycling profile consisting of an initial denaturation at 94°C for 3 min; 37 cycles of denaturation at 94°C for 45 s, annealing at 50°C for 45 s and extension at 72°C for 45 s; followed by a final elongation step at 72°C for 6 min and a hold at 4°C. ..

Polymerase Chain Reaction:

Article Title: Miniature inverted repeat transposable elements in the genome of sugar beet and their impact on gene expression
Article Snippet: Amplification was carried out in a 10 μL total volume containing 20 ng of genomic DNA, 0.5 μM each of forward and reverse primer, 0.25 mM of each dNTP (Thermo Fisher Scientific), 0.5 U Taq DNA polymerase (Thermo Fisher Scientific) and 1 × Taq buffer. .. The PCR amplifications were performed in an Eppendorf MasterCycler Gradient using the following thermal profile: 94 °C (120 s), 30 cycles of 94 °C (30 s), 57 °C (30 s), 68 °C (120 s) and a final step of 68 °C (600 s). ..

Gene Expression:

Article Title: Partial Serotonin Transporter Deficiency Modulates Plasma Metabolome, Arginine-Nitric Oxide Pathway and Emotional Behavior in Mice Exposed to Western Diet.
Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using random primers and the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. .. The reaction was carried out in an Eppendorf Mastercycler® Gradient (Eppendorf https://doi.org/10.3390/metabo16020117 SE, Hamburg, Germany) under the following conditions: 68 ◦C for 5 min, 42 ◦C for 60 min, and 70 ◦C for 10 min. Gene expression analysis was performed using real-time polymerase chain reaction (RT-PCR) with SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA). ..

Article Title: Partial Serotonin Transporter Deficiency Modulates Plasma Metabolome, Arginine-Nitric Oxide Pathway and Emotional Behavior in Mice Exposed to Western Diet
Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using random primers and the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. .. The reaction was carried out in an Eppendorf Mastercycler ® Gradient (Eppendorf SE, Hamburg, Germany) under the following conditions: 68 °C for 5 min, 42 °C for 60 min, and 70 °C for 10 min. Gene expression analysis was performed using real-time polymerase chain reaction (RT-PCR) with SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA). ..

Real-time Polymerase Chain Reaction:

Article Title: Partial Serotonin Transporter Deficiency Modulates Plasma Metabolome, Arginine-Nitric Oxide Pathway and Emotional Behavior in Mice Exposed to Western Diet
Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using random primers and the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. .. The reaction was carried out in an Eppendorf Mastercycler ® Gradient (Eppendorf SE, Hamburg, Germany) under the following conditions: 68 °C for 5 min, 42 °C for 60 min, and 70 °C for 10 min. Gene expression analysis was performed using real-time polymerase chain reaction (RT-PCR) with SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA). ..

SYBR Green Assay:

Article Title: Partial Serotonin Transporter Deficiency Modulates Plasma Metabolome, Arginine-Nitric Oxide Pathway and Emotional Behavior in Mice Exposed to Western Diet
Article Snippet: For cDNA synthesis, 1 μg of total RNA was reverse-transcribed using random primers and the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany), according to the manufacturer’s protocol. .. The reaction was carried out in an Eppendorf Mastercycler ® Gradient (Eppendorf SE, Hamburg, Germany) under the following conditions: 68 °C for 5 min, 42 °C for 60 min, and 70 °C for 10 min. Gene expression analysis was performed using real-time polymerase chain reaction (RT-PCR) with SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA). ..

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